Part:BBa_K4025001

ChiS inter-membrane protein

ChiS is a hybrid sensor kinase originating from the Vibrio cholerae chitin utilization program that is codon-optimised for E. coli. ChiS differs from prototypical two-component signal transduction pathways in that it plays the role of both the sensor kinase and the response regulator. In the presence of chitin (chitin-CBP complex bound to ChiS), ChiS favors dephosphorylation of its receiver domain, allowing it to directly bind and activate Pchb.

ChiS was transformed into plasmid backbone pSB1k03 and a growth curve was constructed to measure cell burden. See: https://2021.igem.org/Team:Lubbock_TTU/Contribution.

As can be seen from the growth curves, the L1 transcriptional unit with ChiS did not overly burdened cell growth– all transformed cells survived. It should be noted; however, that the OD600 readings of all cultures were not standardized prior to inoculation, seen by the varying y-intercepts of all growth curves. Because of this, this data should not be used as quantitative measurements of growth kinetics, but rather a qualitative demonstration of relative growth.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]